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PRODUCTS
(For Research Use Only)
Tuberculosis, caused by Mycobacterium tuberculosis infection, is one of the significant communicable diseases that pose a serious threat to human health. Drug-resistant mutations in Mycobacterium tuberculosis can develop resistance against anti-tuberculosis drugs. Drug-resistant tuberculosis has always been a focal and challenging aspect in tuberculosis prevention and control efforts. It not only requires prolonged and costly treatment but also has a low cure rate, posing a severe threat to the health of society and the general population. Therefore, accurate detection of drug-resistant mutations against anti-tuberculosis drugs is crucial for the diagnosis and treatment of tuberculosis.
The global number of people reported to have been enrolled on treatment for MDR/RR-TB,2015-2021a
DETECTION CONTENT
Tuberculosis drugs | Drug-resistant genes | Analysis projects |
Rifampicin | rpoB | rpoB511-2、rpoB513-1、rpoB513-2、rpoB513-3、 rpoB516-1、rpoB516-2、rpoB516-3、rpoB516-4、 rpoB522-2、rpoB526-1、rpoB526-2、rpoB526-4、 rpoB526-5、rpoB526-6、rpoB531-1、rpoB531-2、 rpoB531-4、rpoB531-5、rpoB533-2 |
Ethambutol | embB | embB306-1、embB306-3、embB406、embB497 |
Isoniazid | inhA | inhA-15 |
katG | katG315-1、katG315-2、katG315-3、katG316-1 | |
Fluoroquinolones | gyrA | gyrA90、gyrA94 |
gyrB | gyrB538 | |
Streptomycin | rpsL | rpsL43、rpsL88 |
Pyrazinamide | pncA | pncA169 |
DETECTION METHOD
Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF-MS) is a novel technique used for the analysis of biological molecules. It involves the formation of a thin co-crystallized film by irradiating the sample with a laser in the presence of a matrix. The matrix absorbs energy from the laser and transfers it to the biological molecules, causing their ionization. The ionized molecules are then accelerated through a flight tube by an electric field, and their time of flight to the detector is used to accurately determine their molecular weight, allowing for precise identification and separation of biomolecules.
PRODUCT INFORMATION
Project name | Technology | Pack Size | Compatible model | Sample type |
Mycobacterium Tuberculosis Drug-resistant Mutation Detection Kit | Nucleic Acid Mass Spectrometry | 40 tests/kit | MassARRAY®DNA Mass Spectrometry Gene Analysis System | paraffin embedded tissue |
DETECTION SIGNIFICANCE
Accurate identification of common drug-resistant gene mutation sites in clinically prevalent Mycobacterium tuberculosis strains, covering six anti-tuberculosis drugs (Rifampin, Ethambutol, Fluoroquinolones, Isoniazid, Streptomycin, and Pyrazinamide), can assist in the clinical treatment of tuberculosis.
FEATURES & ADVANTAGES
1. Technological Advancement: The method combines PCR technology to analyze the nucleic acid molecules of lung infection bacteria, eliminating the need for processes like in vitro culture. This reduces manual operations and allows for a simple and rapid testing process, with results obtained within a day.
2. Stability and Reliability: The detection results are not affected by common lung microorganisms such as Burkholderia cepacia,Pseudomonas aeruginosa, Streptococcus pneumoniae, Legionella pneumophila and Bordetella pertussis.
3. Excellent Specificity: Capable of handling 100 ng of wild-type human genomic DNA without exhibiting non-specific effects.
4. High Sensitivity: It can detect mycobacterial nucleic acid DNA with a content as low as 200 copies in 20 ng of human genomic DNA.
DETECTION PROCESS
1. DNA extraction
2. Template addition
3. Mass spectrometry analysis
4. Data analysis
5. Report generation