（For Research Use Only）

With the widespread use of hematopoietic stem cell transplantation, solid organ transplantation, immunosuppressive agents, chemotherapy drugs, and various invasive procedures, the incidence of invasive fungal infections in the lungs is gradually increasing. Patients with pulmonary fungal infections often lack specific symptoms, and early diagnosis is challenging. The condition is easily masked by underlying diseases, leading to misdiagnosis, missed diagnosis, and delayed treatment, resulting in a high mortality rate. Therefore, accurate detection of invasive fungal infections in lung tissue is crucial for the diagnosis and treatment of the disease.

DETECTION CONTENT

DETECTION METHOD

Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF-MS) is a novel technique used for the analysis of biological molecules. It involves the formation of a thin co-crystallized film by irradiating the sample with a laser in the presence of a matrix. The matrix absorbs energy from the laser and transfers it to the biological molecules, causing their ionization. The ionized molecules are then accelerated through a flight tube by an electric field, and their time of flight to the detector is used to accurately determine their molecular weight, allowing for precise identification and separation of biomolecules.

PRODUCT INFORMATION

DETECTION SIGNIFICANCE

Accurate identification of the 30 common invasive fungal species in clinical settings provides a clear understanding of the pathogenic organisms and offers a scientific basis for anti-infective treatment.

FEATURES & ADVANTAGES

1. Technological Advancement: The method combines PCR technology to analyze the nucleic acid molecules of lung infection bacteria, eliminating the need for processes like in vitro culture. This reduces manual operations and allows for a simple and rapid testing process, with results obtained within a day.

2. Stability and Reliability: The detection results are not affected by common lung microorganisms such as Burkholderia cepacia，Pseudomonas aeruginosa, Streptococcus pneumoniae, Legionella pneumophila and Bordetella pertussis.

3. Excellent Specificity: Capable of handling 100 ng of wild-type human genomic DNA without exhibiting non-specific effects.

4. High Sensitivity: It can detect mycobacterial nucleic acid DNA with a content as low as 200 copies in 20 ng of human genomic DNA.

DETECTION PROCESS

1. DNA Extraction

2. Template Addition

3. Mass Spectrometry Analysis

4. Data Analysis

5. Report generation