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(Real-time PCR assay)
GENE MUTATION AND TUMOR
The protein encoded by the MLH1 gene is involved in DNA mismatch repair. The methylation of the MLH1 promoter is commonly found in sporadic microsatellite instability tumors including colorectal cancer and endometrial cancer, which is related to the loss of MLH1 protein expression. Methylation of the MLH1 gene promoter is rarely found in Lynch syndrome (hereditary non-polyposis colorectal cancer, HNPCC). Therefore, MLH1 gene methylation analysis can be used to distinguish sporadic and hereditary colorectal cancer and endometrial cancer in highly unstable microsatellite tumors. In endometrial cancer, MLH1 gene methylation is highly aggressive and need to be treated positively.
MLH1 gene methylation.
MLH1 gene methylation detection kit
DETECTION SIGNIFICANCE &Applicable People
1. Genetic risk warning: Genetic risk prompt: MLH1 gene methylation detection can exclude familial inheritance of tumors. If MMR function is lost due to MLH1 gene methylation, familial inheritance is not high, it may be suspected that it is Lynch syndrome with familial heredity, and the risk of cancer in the family increases.
2. Cancer classification and treatment guidance: If MLH1 is methylated, the expression of MMR protein in the cancer tissue is lost, which indicates that it is a sporadic cancer. If this happens in colorectal cancer and endometrial cancer often indicate a poor prognosis and active treatment is recommended.
Applicable people: Patients with colorectal cancer and endometrial cancer.
MLH1 gene methylation
The positive methylation of the MLH1 gene promoter region indicates that the loss of MLH1 gene expression is due to
epigenetic modification rather than genetic mutation, which suggest a lower possibility of Lynch syndrome. It is recomm-ended to combine clinical information and family history for
FEATURES & ADVANTAGES
1.Accuracy and Reliability:Use pre-loaded PCR tube to effectively avoid cross-contamination.
2.High sensitivity:Sensitivity can detect as low as 1% gene methylation status.
3.Easy of use:Based on technology PAP-ARMS®, one step detection in 90 mins.
4.Great versatility:Validated on the most common qPCR machines with stable results.
1.Nucleic Acid Extraction
3.Set up qPCR