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PRODUCTS
(Real-time PCR assay)
CE-IVD
GENE MUTATION AND TUMOR
The protein encoded by the MLH1 gene is involved in DNA mismatch repair. The methylation of the MLH1 promoter is commonly found in sporadic microsatellite instability tumors including colorectal cancer and endometrial cancer, which is related to the loss of MLH1 protein expression. Methylation of the MLH1 gene promoter is rarely found in Lynch syndrome (hereditary non-polyposis colorectal cancer, HNPCC). Therefore, MLH1 gene methylation analysis can be used to distinguish sporadic and hereditary colorectal cancer and endometrial cancer in highly unstable microsatellite tumors. In endometrial cancer, MLH1 gene methylation is highly aggressive and need to be treated positively.
colorectalcancer screening
Endometrial cancerscreening
DETECTION GENE
MLH1 gene methylation.
PRODUCT INFORMATION
| Product Name | Core Technology | Pack Size | Instruments Validated | Sample Type |
MLH1 gene methylation detection kit | PAP-ARMS® | 24 test/kit | Stratagene Mx3000P™, ABI7500 etc | Tumor Tissue（Including paraffin sections） |
DETECTION SIGNIFICANCE &Applicable People
1. Genetic risk warning: Genetic risk prompt: MLH1 gene methylation detection can exclude familial inheritance of tumors. If MMR function is lost due to MLH1 gene methylation, familial inheritance is not high, it may be suspected that it is Lynch syndrome with familial heredity, and the risk of cancer in the family increases.
2. Cancer classification and treatment guidance: If MLH1 is methylated, the expression of MMR protein in the cancer tissue is lost, which indicates that it is a sporadic cancer. If this happens in colorectal cancer and endometrial cancer often indicate a poor prognosis and active treatment is recommended.
Applicable people: Patients with colorectal cancer and endometrial cancer.
DETECTION RESULTS
Detection Item | Detection Results | Result Analysis |
MLH1 gene methylation detection kit | Positive | The positive methylation of the MLH1 gene promoter region indicates that the loss of MLH1 gene expression is due to epigenetic modification rather than genetic mutation, which suggest a lower possibility of Lynch syndrome. It is recomm-ended to combine clinical information and family history for comprehensive analysis. |
FEATURES & ADVANTAGES
1.Accuracy and Reliability:Use pre-loaded PCR tube to effectively avoid cross-contamination.
2.High sensitivity:Sensitivity can detect as low as 1% gene methylation status.
3.Easy of use:Based on technology PAP-ARMS®, one step detection in 90 mins.
4.Great versatility:Validated on the most common qPCR machines with stable results.
DETECTION PROCESS
1.Nucleic Acid Extraction
2.Modification
3.Set up qPCR
4.Amplification
5.Data Analysis
Company Name:
XIAMEN SPACEGEN CO.,LTD
Address Of The Company:
NO.2041,XizhouRoad,Xike Town,
Tong'an District,Xiamen City,
Fujian Province, China
Contact Information:+86-592-7578317
Postal Code : 361100
spacegen@ispacegen.com
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