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(Real Time PCR)
CE-IVD
Background（Disease，Gene，etc.）
The protein encoded by the MLH1 gene is involved in DNA mismatch repair.The methylation of the MLH1 promoter is commonly found in sporadicmicrosatellite instability tumors including colorectal cancer and endometrialcancer, which is related to the loss of MLH1 protein expression. Methylationof the MLH1 gene promoter is rarely found in Lynch syndrome (hereditarynon-polyposis colorectal cancer, HNPCC). Therefore, MLH1 gene methylationanalysis can be used to distinguish sporadic and hereditary colorectal cancerand endometrial cancer in highly unstable microsatellite tumors. In endometrialcancer, MLH1 gene methylation is highly aggressive and need to be treatedpositively.
colorectalcancer screening
Endometrial cancerscreening
Detection Item
MLH1 gene methylation
Performance Parameter
Product Name | Core Technology | Product Specification | Compatible Instruments | Sample Type |
Human MLH1 gene methylation detection kit | PAP-ARMS® | 24 test/kit | Stratagene Mx3000P™, ABI7500, ABI7300Plus, SLAN-96P/SLAN-48P | Tumor Tissue（including paraffin sections） |
DetectionSignificance /Applicable People
Detection Significance
① Geneticrisk warning:The MLHI gene methylation detection has a suggestive effect on the familyheredity of the tumor. If the MMR function is missing due to the methylation ofthe MLH1 gene, the family heritability is not high, otherwise it may besuspected of family hereditary Lynch syndrome. As the risk of cancer isincreased, further germline sequencing analysis and genetic counseling arerecommended.
②Cancerclassification and treatment guidance: If MLH1 is methylated, the expression of MMR protein in the cancertissue is lost, which indicates that it is a sporadic cancer. If this happensin colorectal cancer and endometrial cancer often indicate a poor prognosis andactive treatment is recommended.
Applicable people: patients with colorectalcancer and endometrial cancer.
Detection Results
Detection Item | Detection Results | Result Analysis |
Human MLH1 gene methylation detection kit | positive | The positive methylation of the MLH1 gene promoter region indicates that the loss of MLH1 gene expression is due to epigenetic modification rather than genetic mutation, which suggest a lower possibility of Lynch syndrome. It is recommended to combine clinical information and family history for comprehensive analysis. |
Product Advantage
Strongspecificity:Closed tube detection, avoid contamination.
High sensitivity: The detection can reach as low as 1% in 10 ng DNA.
Easyoperations: Onestep operation, only 90 minutes to finish the detection.
Good reproducibility: Can be performed in the general PCR Labs. Results with goodreproducibility can be easily obtained without special training.
Advanced technologies: R&D based on independent patented PAP-ARMS technology.
Detection Process
Company Name:
XIAMEN SPACEGEN CO.,LTD
Address Of The Company:
NO.2041,XizhouRoad,Xike Town,
Tong'an District,Xiamen City,
Fujian Province, China
Contact Information:+86-592-7578317
Postal Code : 361100
spacegen@ispacegen.com
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