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（Real-time PCR assay）
PAX1 GENE METHYLATION ASSOCIATED WITH CERVICALCANCER
Abnormal methylation of PAX1 gene can reflect the pathological process of cervical cancer and precancerous lesions of different stages. It is closely related to the occurrence and progression of cervical cancer. Its detection of precancerous lesions or cervical cancer can be early than conventional Thinprep cytologic test. (TCT). PAX1 gene methylation detection combined with HPV and TCT detection will help increasing the detection rate of cervical cancer.
CERVICAL CANCER RELATED SCREENING TECHNOLOGY
smear ( Pap Smear)
Scrape the epithelial cells in the cervix
with a scraper, apply evenly on the glass
slide,fix and stain.
Lower cost: Due to human factors in the
acquisition, production, and reading, the
results are limited.
This method has gradually been eliminated.
The obtained exfoliated cells from cervical secretions are collected and immersed
in liquid cell processing reagents, and
then exfoliated cell slices are made. After staining, the cell morphology of the
cervix can be checked by manual
observation and analysis to diagnose
cervical cancerand itsprecancerous
Convenient sampling and breakthrough in
production technology; Need manual
observation and analysis of cell morphology.
Easy to be interfered by human factors.
The diagnosis rate of atypical squamous cell
smear is not high, often unable to detect
Use DNA amplification and hybridization
technology to detect cervical cell
samples for HPV infection status and
typing, Mainly focus on the typing of
high-risk HPV (hr-HPV) such as 16 and 18.
High throughput and sensitivity.Can detect
multiple infections, suitable for large-scale
clinical screening.Detection of viruses instead
of individual genes. Only early warning rather
There is a possibility for false positive.
Detect cervical smear samples for the
high-level sensitivity and specify of PAX1
Accurate screening at the individual genetic
level, safe and reliable.High throughput and
sensitivity, strong specificity.The operation is
automatic and good repeatability.
THREE APPROACHES TO CERVICAL CANCER SCREENING AND FUTURE TESTS
Nucleic acid amplification tests
» high-risk HPV DNA/NAAT
» HPV antibodies
Conventional Pap smear
Liquid-based cytology (LBC)
Dual staining to identify p16 and
Visual inspection with acetic acid or with
Lugol’s iodine (VIA/VILI)
» naked eye
» magnified by
colposcope or camera
evaluation of digital images
WHO guideline for screening and treatment of cervical pre-cancer lesions for cervical cancer prevention, second edition
DETECTION DESIGN OF PAX1 METHYLATION
This kit uses fluorescent PCR amplification technology to qualitatively detect the methylation of PAX1 gene specific sites in DNA samples. Combines with HPV and TCT testing to provide a reference for accurate cervical cancer screening.
● Cervical cancer screening or regular assessment
● HPV positive carrier
● Patients with abnormal cervical smear test
● Cervical spine incision, cervical surgery, patient monitoring after
radiotherapy and chemotherapy
● People with unexplained vaginal bleeding
● New biomarkers of cervical cancer
● High specificity and sensitivity
● The operation is automatic, simple ,fast, and with good repeatability
● Independent screening, safe and reliable
Human PAX1 Gene
Methylation Detection Kit
1.Nucleic Acid Extraction
3.Set up qPCR