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B-raf Gene Mutation Detection Kit

(Real-time PCR assay)

Research Use Only 



BRAF gene plays an important role in signal transduction involving cell proliferation, by regulating various kinds of cell physiological activities, such as growth, division , death etc.


BRAF gene is the second transmitter in the EGFR signaling pathway. When BRAF gene mutations occurs, the signal pathway will be permanently auto-activated, resulting in excessive cell proliferation and differentiation, thus, which may lead to EGFR-TKI drug ineffectiveness. Mutations of BRAF occurred in many cancers such as malignant melanoma, colorectal cancer, lung cancer, thyroid cancer, liver cancer,etc.


The NCCN Guidelines for Colorectal Cancer (2019 Edition) recommends that all patients with metastatic colorectal cancer can be genotyped for B-raf and K-ras mutations.



B-raf Gene Mutation &Drugs




DETECTED GENES

GeneCancer TypeGene StatusTarget DrugDrug Indications
B-raf V600ENSCLCmutation type

Trametinib + Dabrafenib/

 Vemurafenib

resistant

CRC

mutation type

Encorafenib+ Cetuximab

/ Panitumumab

sensitive
wild typeCetuximab/ Panitumumabsensitive
Melanomamutation type

 Vemurafenib+ Cobimetinib/ 

Binimetinib/ Trametinib + Dabrafenib

sensitive



PRODUCT INFORMATION

Product Name

Core

Technology

Pack SizeInstruments ValidatedSample TypeModel

B-raf Gene Mutation

Detection Kit

PAP-ARMS®20 Tests/Kit

Stratagene Mx3000P™, 

ABI 7500 etc.

Tumor tissue,

Peripheral blood,

Pleural and ascites

SPG-BRAF001R


Detection Significance

1. Patients with suspected Lynch syndrome can be  Screening  by BRAF genetic detection.

2. Can be used for the identification of diagnosis for thyroid cancer, assessing the prognosis for malignant melanoma and colorectal cancer.

3. For patients treated with BRAF and EGFR inhibitors, detection of BRAF gene mutation status can be used to adjust diagnosis and treatments.



FEATURES & ADVANTAGES

1.Accuracy and Reliability: Use pre-load PCR tube to effectively avoid cross-contamination.

2.High Sensitivity: Sensitivity can detect V600E mutations as low as 1%in 10 ng DNA.

3.Ease of Use: Based on technology PAP-ARMS®, one step detection in 90 mins. 

4.Great versatility: Validated on the most common qPCR machines with stable results.



DETECTION PROCESS 

1.Nucleic Acid Extraction

2.Set up qPCR

3.Amplification

Data Analysis


The product that is intended for research use only.